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KMID : 1134820060350081010
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Journal of the Korean Society of Food Science and Nutrition
2006 Volume.35 No. 8 p.1010 ~ p.1015
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Effect of Mixture of p-Phenylenediamine with Hydrogen Peroxide to Rat Skin
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Lee Sang-Hee
Lee Sang-Il
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Abstract
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p-Pheylenediamimine (PPD) is one of hair dye’s ingredients, and the mixture of PPD with hydrogen peroxide is generally used to dye hair at beauty shop. This study is conducted to investigate the effect of oxidized PPD on rat skin. 6% hydrogen peroxide, PPD (5% PPD in 2% NH₄OH) or the mixture (isovolumed mixture of 5% PPD and 6% hydrogen peroxide in 2% NH₄OH) was applied to rat skin (25 ㎎/16.5 ㎠) five times every other day. The activity of acid phosphatase (ACP) was more increased in the mixture of PPD with hydrogen peroxide applied group than PPD applied group. Furthermore, the activity of glucose 6-phosphatase (G6Pase) in the mixture of PPD with hydrogen peroxide applied group showed higher decreasing rate than that of PPD applied group. In histopathological findings, the mixed PPD with hydrogen peroxide applied group showed more thickening of epithelium, increased numbers of dermal fibroblasts, and the dilatation of dermal capillaries than PPD applied group. The significant increasing of xanthine oxidase (XO) activity was determined in mixture of PPD with hydrogen peroxide applied group compared with PPD applied group. However, reactive oxygen species (ROS) scavenging system, the activities of superoxide dismutase (SOD) and glutathione S-transferase (GST) were more significantly decreased in mixed PPD with hydrogen peroxide applied groups than in PPD applied group. In conclusion, topical application with the mixture of PPD with hydrogen peroxide compared with PPD application resulted in imbalance with ROS generating and scavenging which probably led to severe skin injury.
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KEYWORD
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hydrogen peroxide, p-phenylenediamine, rat skin, reactive oxygen species(ROS)
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